Dehydroepiandrosterone (DHEA) was first identified by Dr. Adolf Butenandt, a German biochemist, in 1931. Dr. Butenandt was awarded the Nobel Prize for chemistry in 1939. DHEA is primarily produced in the adrenal glands, gonads and skin. DHEA is a naturally occurring pro-hormone or hormone precursor. In 1944, the sulfated form of DHEA was isolated (DHEA-S).
Clinical research revealed that humans make increasing amounts of DHEA from birth to about age 25. DHEA is the most abundant hormone precursor. Men and women produce maximum levels during the years of reproductive prime; but then, the amount of DHEA produced steadily declines at a rate of about 2% per year from about age 25-30 until death. Medical research performed in the 1960’s and 1970’s demonstrated improvements in physical and psychological well-being with regard to bone strength, improved sleep, as well as cardiovascular benefits.
Medical research studies published in the 1980’s and 1990’s continued to confirm that DHEA supplementation was beneficial for numerous biological and cognitive health outcomes.  In 1994 Dr. Samuel Yen, a world-renown endocrinologist at the University of California at San Diego and subject matter expert, concluded that DHEA may help people age more gracefully, sleep better at night, and have a greater sense of well-being. The highly respected hormone expert reported this at the New York Academy of Sciences in June 1995. Dr. Yen’s conducted research which demonstrated that DHEA replacement activates the immune system, increases well-being, boosts energy levels, improves mood, and allows a greater ability to cope with stressful events. 
In the 1990’s through 2008, Dr. Fernand Labrie, the principal investigator at University of Toronto Medical School in Canada, published important studies demonstrating that DHEA, and it’s use as a cream, yields significant health benefits. In these studies, Dr. Labrie’s preferred method of use was DHEA applied as a specially formulated cream.
Dr. Fernand Labrie’s study results demonstrate that when DHEA is taken orally as a pill, spray or troche, most DHEA is eliminated by the first-pass effect of the liver, thereby primarily increasing only DHEA-sulfate (DHEA-S) levels in the bloodstream – not free DHEA, which is what serves as a base for the sex hormones. DHEA-S is not readily converted into free DHEA as was found by Dr. Fabien Hammer et al in his study titled “No Evidence for hepatic conversion of dehydroepiandrosterone (DHEA) sulfate to DHEA: In vivo and in vitro studies” (www.ncbi.nlm.nih.gov/pubmed/15755854).  Thus, DHEA pills yield little to no noticeable benefits the user can feel. However, a properly made 5% (50 mg/cc) DHEA cream does provide the user noticeable benefits. Medical research findings support this as do the experience of thousands of Twist 25 users.
Research shows that it is in the skin (dermis), the largest organ of the human body, where much DHEA is metabolized, especially after menopause or andropause. In fact, excellent research done by Dr. Fernand Labrie et al (See “Adrenal androgens and intracrinology” www.ncbi.nlm.nih.gov/pubmed/15635498 ) determined that for postmenopausal women, more than 88 % of hormones are metabolized in the skin, and for men more than 50%. 
Twist 25 DHEA cream provides bioidentical DHEA via a specially designed, compounded cream base made with coconut oil, so DHEA is absorbed in the dermis yielding noticeable benefits within just a few weeks.
Twist 25 DHEA cream is absorbed and processed in the skin, not through the gut and liver.
One full pump press of Twist 25 cream contains 25 mg DHEA. The cream is applied daily on thin hairless skin. Twist 25 cream is best applied daily on the inner arm between wrist and elbow; and rubbed in with the arm in a circular motion on the side or abdomen until it is absorbed. This takes about 10 seconds and leaves no odor or residue.
Twist 25 cream was developed by Dr. John R. Woodward, a board-certified medical doctor who specializes in bioidentical hormone replacement therapy. Twist 25 DHEA cream has been used and tested for over 30 years. It is safe and effective. The favorable user outcomes and lack of adverse results speak for themselves.
 Guber HA, Farag AF, Lo J, Sharp J. Evaluation of Endocrine Function. Henry’s Clinical Diagnosis and Management by Laboratory Methods. 21st edition Philadelphia, PA: W.B. Saunders Company; 2006: Chapter 24
 Fernand Labrie, Alain Belanger, Rene Berube, et al. University of Toronto Medical School. The Journal of Steroid Biochemistry and Molecular Biology Vol 103(2), Issue 2, Feb 2007, Pgs 178-188
 Fabian Hammer, Sandra Subtil, Philipp Lux, Christiane Maser-Gluth, Paul M. Stewart, Bruno Allolio and Wiebke Arlt. No Evidence for Hepatic Conversion of Dehydroepiandrosterone (DHEA) Sulfate to DHEA: In Vivo and in Vitro Studies. Journal of Clinical Endocrinology and Metabolism Vol. 90, No. 6 3600-3605
 Labrie, Fernand, Belanger, Alain, Belanger, Patrick, Berube, Rene, Martel C, Cusan L, Gomez, J, et al “Metabolism of DHEA in postmenopausal women following percutaneous administration”. ScienceDirect Pubmed. Journal of Steroid Biochemistry and Molecular Biology 103 (2007) 178-188. http://www.ncbi.nlm.nih.gov/pubmed/17084625
 Labrie F, Belanger A, Labrie C, Candas B, Cusan L, Gomez JL. “Bioavailability and metabolism of oral and percutaneous dehydroepiandrosterone in postmenopausal women”. Journal of Steroid Biochemistry and Molecular Biology. Jun 8 2007 PubMed https://www.ncbi.nlm.nih.gov/pubmed/17627814